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Cardiomyocyte

Cardiomyocyte

A cardiomyocyte, one of the cells responsible for contractile force in the heart, with labeled hormone receptors, histone markers, and myosin.

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Zebrafish Angiogenesis

Zebrafish Angiogenesis

GFP labeled endothelial cells identify zebrafish vasculature perturbations.

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Mitochondria, Nuclei, and Actin.

Mitochondria, Nuclei, and Actin.

Arbitrary pixel sizes in laser scanning microscopes permit low-magnification (40x), yet high-resolution (approximately 200 nm), imaging.

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Vesicular Trafficking in Hippocampal Neurons

Vesicular Trafficking in Hippocampal Neurons

Fluorescently labeled dyes permit tracking of vesicular cargo upon stimulation of hippocampal neurons.

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Salmonella Induced Filaments

Salmonella Induced Filaments

Salmonella, upon internalization by mammalian host cells, induces an intracellular niche that includes highly dynamic filaments that can be observed with a split-GFP imaging system.

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High-Content Imaging

High-Content Imaging

Doug Chapnick, recent PhD graduate, setting up a high-content imaging experiment on the Molecular Devices ImageXpress.

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Imaging on Confocal

Imaging on Confocal

Ali & Sarah imaging intracellular pathogen Salmonella dynamics on the Nikon Confocal.

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Confocal Optics

Confocal Optics

It's always impressive to see what takes place behind the scenes in a microscope. Here, pictured are the optics which using a series of dichroic mirrors combine all of the various lasers into a single optical path, the acoustic-optic tunable filter (center, red) which selects the wavelength of light on the microsecond timescale, and the motorized mirror that switches between the TIRF and confocal paths. After selecting which imaging mode (TIRF/Confocal), the beams are then focused into a single-mode optical fiber using the optical assembly on the front right and left.

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Actin Filaments in Cardiomyocytes

Actin Filaments in Cardiomyocytes

Actin filaments in cardiomyocytes stained with phalloidin and imaged with a 40x objective.

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Actin, Myosin, and Nuclear Staining

Actin, Myosin, and Nuclear Staining

Actin, Myosin, and Nuclear Staining

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Before Autophagy

Before Autophagy

A sequence of events leading to autophagy are provided by imaging cells with labeled mitochondria, Parkin, and LC3.

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After Autophagy

After Autophagy

A sequence of events leading to autophagy are provided by imaging cells with labeled mitochondria, Parkin, and LC3.

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RNA FISH

RNA FISH

Imaging RNA molecules within the cellular environment can be a challenging process. Here, the nucleus is stained with DAPI (blue), and specific RNA molecules are labeled (green). The unique sub-cellular distribution of this particular RNA, being both cytoplasmic and the nucleolar, provides immediate insight into the cellular biology.

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